Glycan Array Experiment: primscreen_6592
Glycan Array ID
:
primscreen_6592
Sample Name
:
MISTRG mouse sera 1
Sample Category
:
Antibodies
Investigator:
:
Peng George Wang
Download Data:
Sample Information:
Sample Name
:
MISTRG mice sera-1
Sample Name (serum sample)
:
MISTRG mice sera-1
Experiment title and rationale for assay
:
screening for antibody of anti-glycan in MISTRG mice (humanized mice)
Species in which your serum originated (human, mouse, etc.) with Scientific Name and Strain: Type Age and Gender (if Human , type 1.Race/ethnicity: 2.ABO Blood Type: 3. Diagnosis/disease:)
:
MISTRG mice
Serum collection and processing information: type 1.Anti-coagulant: 2.Dilution in what diluent: 3.Condition of storage: 4.Time in storage: 5.Post collection processing (IgG depletion, ammonium sulfate precipitated, etc.)
:
No dilution
Experiment Information:
Status:
:
Public
Title/Project Description:
:
The purpose of this request is to studying \u0093human-type\u0094 immune responses to glycan antigens using the humanized mouse system (HIS), which is a mouse strain that has an immune system genetically modified to resemble the human immune system (see Balazs et al (2014) Nature Med. 20(3):296-300).
Associated Resource Requests:
:
cfg_rRequest_3057
Glycan Array Version:
:
PA_v52
Protocol Used:
:
Protocol Polyclonal Antibodies/Serum
Replicate Number:
:
1
Result Nature:
:
Inconclusive
Protein/Sample Concentration:
:
Information not entered/not applicable.
Experiment Date [yyyy-mm-dd]:
:
2014-07-25
Supplying Investigator
:
Peng George Wang
Specific Assay Information
:
Serum sample was diluted 1:50 in standard binding buffer and was detected by anti-mouse IgG-488 followed by anti-mouse IgM-488 (Invitrogen, 5 ug/ml).
Annotation
:
MISTRG mouse sera 1
Investigator Sample Analyzed
:
_