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The three selectins (P-selectin, L-selectin, and E-selectin) have related and sometimes overlapping functions in cell adhesion and mediate some of the best characterized glycan-dependent cell adhesion events. Of these three C-type lectins, the target ligand of P-selectin, P-selectin glycoprotein ligand 1 (PSGL-1), is the best understood. Thus, P-selectin is used here to represent all three selectins.


CFG Participating Investigators contributing to the understanding of this paradigm

Selectin research was already at a relatively mature stage when the CFG began. Early PI work included structural studies, extensive analysis of leukocyte adhesion to endothelia in vivo, and characterization of knockout mice to demonstrate physiological function. In addition to submitting samples for glycan array analysis, PIs have been involved in analyzing selectin expression under different conditions, including in knockout mice lacking enzymes for making target ligands.

  • PIs working on P-selectin include: Hans-Peter Altevogt, Bruce Bochner, Pi-Wan Cheng, Richard Cummings, Robert Fuhlbrigge, Minoru Fukuda, Geoff Kansas, Klaus Ley, John Lowe, Rodger McEver, Steve Rosen, Ron Schnaar, Karen Snapp, Lloyd Stoolman, Martin Wild, Hermann Ziltener
  • Non-PIs who have used CFG resources to study P-selectin include: Roland Contreras, Leonard Seymour

Progress toward understanding this GBP paradigm

Carbohydrate ligands

Cellular expression


Biological roles of GBP-ligand interaction

CFG resources used in investigations

The best examples of CFG contributions to this paradigm are described below, with links to specific data sets. For a complete list of CFG data and resources relating to this paradigm, see the CFG database search results for P-selectin.

Glycan profiling

The glycans on the main target for P-selectin, P-selectin glycoprotein ligand 1 (PSGL-1), were analyzed.

Glycogene microarray

Regulation of P-selectin expression was analyzed under multiple conditions.

Knockout mouse lines

The phenotype of PSGL-1 knockout mice was analyzed by the CFG.

Glycan array

The comparative binding specificities of human and mouse selectins were analyzed.

Related GBPs

L-selectin and E-selectin


  • Mitoma J, Miyazaki T, Sutton-Smith M, Suzuki M, Saito H, Yeh JC, Kawano T, Hindsgaul O, Seeberger PH, Panico M, Haslam SM, Morris HR, Cummings RD, Dell A, Fukuda M (2009) The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells. Glycoconj J 26, 511-523.
  • Kawar ZS, Johnson TK, Natunen S, Lowe JB, Cummings RD (2008) PSGL-1 from the murine leukocytic cell line WEHI-3 is enriched for core 2-based O-glycans with sialyl Lewis x antigen. Glycobiology 18, 441-446.
  • Mitoma J, Bao X, Petryanik B, Schaerli P, Gauguet JM, Yu SY, Kawashima H, Saito H, Ohtsubo K, Marth JD, Khoo KH, von Andrian UH, Lowe JB, Fukuda M (2007) Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment. Nat Immunol 8, 409-418.
  • Veerman KM, Williams MJ, Uchimura K, Singer MS, Merzaban JS, Naus S, Carlow DA, Owen P, Rivera-Nieves J, Rosen SD, Ziltener HJ (2007) Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs. Nat Immunol 8, 532-539.
  • Chen S, Kawashima H, Lowe JB, Lanier LL, Fukuda M (2005) Suppression of tumor formation in lymph nodes by L-selectin-mediated natural killer cell recruitment. J Exp Med 202, 1679-1689.
  • Kawashima H, Petryniak B, Hiraoka N, Mitoma J, Huckaby V, Nakayama J, Uchimura K, Kadomatsu K, Muramatsu T, Lowe JB, Fukuda M (2005) N-acetylglucosamine-6-O-sulfotransferases 1 and 2 cooperatively control lymphocyte homing through L-selectin ligand biosynthesis in high endothelial venules. Nat Immunol 6, 1096-1104.
  • Piccio L, Rossi B, Colantonio L, Grenningloh R, Gho A, Ottoboni L, Homeister JW, Scarpini E, Martinello M, Laudanna C, DAmbrosio D, Lowe JB, (2005) Constantin G Efficient recruitment of lymphocytes in inflamed brain venules requires expression of cutaneous lymphocyte antigen and fucosyltransferase-VII. J Immunol 174, 5805-5813.
  • Homeister JW, Daugherty A, Lowe JB (2004) α(1,3)Fucosyltransferases FucT-IV and FucT-VII control susceptibility to atherosclerosis in apolipoprotein E -/- mice. Arterioscler Thromb Vasc Biol 24, 1897-1903.
  • Lowe JB (2003) Glycan-dependent leukocyte adhesion and recruitment in inflammation Curr Opin Cell Biol 15, 531-538.
  • Smith PL, Myers JT, Rogers CE, Zhou L, Petryniak B, Becher DJ, Homeister JW, Lowe JB (2002) Conditional control of selectin ligand expression and global fucosylation events in mice with a targeted mutation at the FX locus. J Cell Biol 158, 801-815.


The CFG is grateful to the following PIs for their contributions to this wiki page: Kurt Drickamer, Minoru Fukuda, Yvette van Kooyk

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