Galectin-1

From CFGparadigms

(Difference between revisions)
Jump to: navigation, search
(Knockout mouse lines)
Line 34: Line 34:
=== Glycan profiling ===
=== Glycan profiling ===
 +
 +
This is a test.
<br>
<br>

Revision as of 16:57, 13 May 2010

Galectin-1 is the best-studied of the prototypic galectins. The crystal structure of Galectin-1 is known, and was the first crystal structure identified for a prototypic galectin.
In addition, Galectin-1...

  • was the first prototypic galectin for which a function was identified.
  • was the first prototypic galectin that was genetically ablated in mice; galectin-1 knockout mice have distinct phenotypes, including aberrant T lymphocyte development and increased susceptibility to autoimmune disease.
  • is the only prototypic galectin that has been administered in animal models of disease to assess therapeutic potential.
  • has novel dynamics and functions regarding it oxidized versus reduced status, as well as its dimerization status[1][2].
  • binds novel N- and O-glycan determinants that are involved in cell signaling[2][3][4][5][6].
  • demonstrates novel distributions in muscle cells versus non-muscle cells[7].
  • ligands are modulated by their differential sialylation that is also associated with glycoprotein positioning in membranes[8].
  • is involved in lymphocyte trafficking and leukocyte recruitment[5][9].


Contents

CFG Participating Investigators contributing to the understanding of this paradigm

CFG Participating Investigators (PIs) contributing to the understanding of Galectin-1 include: Linda Baum, C. Fred Brewer, Richard Cummings, Anne Dell, Ten Feizi, M.G. Finn, Thomas Gerken, Benhur Lee, J. Michael Pierce, Mauro Perretti, Gabriel Rabinovich, James Rini, Sachiko Sato, Gerald Schwarting, Pamela Stanley, Victor Thijssen, Gerardo Vasta, John Wang

Progress toward understanding this GBP paradigm

Carbohydrate ligands


Cellular expression


Structure


Biological roles of GBP-ligand interaction


CFG resources used in investigations

The best examples of CFG contributions to this paradigm are described below, with links to specific data sets. For a complete list of CFG data and resources relating to this paradigm, see the CFG database search results for Galectin-1.

Glycan profiling

This is a test.


Glycogene microarray


Knockout mouse lines

CFG-generated Galectin-1 knockout mice have been used to study the biological functions of this paradigm GBP. The phenotype of Galectin-1 knockout mice was analyzed by the CFG.

Glycan array

Investigators have used carbohydrate compounds and glycan microarrays to study ligand binding specificity of Galectin-1.

Related GBPs

Galectins-2, -5, -7, -10, -11, -13, and -14

References

  1. Stowell SR, et al. Ligand reduces galectin-1 sensitivity to oxidative inactivation by enhancing dimer formation. J Biol Chem 284, 4989-4999 (2009).
  2. 2.0 2.1 Leppanen A, Stowell S, Blixt O, Cummings RD. Dimeric galectin-1 binds with high affinity to alpha2,3-sialylated and non-sialylated terminal N-acetyllactosamine units on surface-bound extended glycans. J Biol Chem 280, 5549-5562 (2005).
  3. Earl LA, Bi S, Baum LG. N- and O-glycans modulate galectin-1 binding, CD45 signaling, and T cell death. J Biol Chem 285, 2232-2244 (2010).
  4. Song X, et al. Novel fluorescent glycan microarray strategy reveals ligands for galectins. Chem Biol 16, 36-47 (2009).
  5. 5.0 5.1 Cooper D, Norling LV, Perretti M. Novel insights into the inhibitory effects of Galectin-1 on neutrophil recruitment under flow. J Leukoc Biol 83, 1459-1466 (2008).
  6. Stillman BN, et al. Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. J Immunol 176, 778-789 (2006).
  7. Dias-Baruffi M, et al. Differential expression of immunomodulatory galectin-1 in peripheral leukocytes and adult tissues and its cytosolic organization in striated muscle. Glycobiology In Press. (2010).
  8. Cha SK, et al. Removal of sialic acid involving Klotho causes cell-surface retention of TRPV5 channel via binding to galectin-1. Proc Natl Acad Sci U S A 105, 9805-9810 (2008).
  9. Norling LV, Sampaio AL, Cooper D, Perretti M. Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking. Faseb J 22, 682-690 (2008).

Acknowledgements

The CFG is grateful to the following PIs for their contributions to this wiki page: Linda Baum, Richard Cummings

Personal tools