Galectin-1
From CFGparadigms
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=== Knockout mouse lines === | === Knockout mouse lines === | ||
| - | CFG-generated galectin-1 knockout mice have been used to study the biological functions of this paradigm GBP. The phenotype of galectin-1 knockout mice was analyzed by the CFG. | + | CFG-generated [http://www.functionalglycomics.org/static/consortium/resources/resourcecoref6.shtml galectin-1 knockout mice] have been used to study the biological functions of this paradigm GBP. The [http://www.functionalglycomics.org/glycomics/publicdata/phenotyping.jsp phenotype] of galectin-1 knockout mice was analyzed by the CFG. |
=== Glycan array === | === Glycan array === | ||
Revision as of 23:20, 1 April 2010
Galectin-1 is the best-studied of the prototypic galectins. The crystal structure of galectin-1 is known, and was the first crystal structure identified for a prototypic galectin.
In addition, galectin-1...
- was the first prototypic galectin for which a function was identified.
- was the first prototypic galectin that was genetically ablated in mice; galectin-1 knockout mice have distinct phenotypes, including aberrant T lymphocyte development and increased susceptibility to autoimmune disease.
- is the only prototypic galectin that has been administered in animal models of disease to assess therapeutic potential.
- has novel dynamics and functions regarding it oxidized versus reduced status, as well as its dimerization status[1][2].
- binds novel N- and O-glycan determinants that are involved in cell signaling[2][3][4][5][6].
- demonstrates novel distributions in muscle cells versus non-muscle cells[7].
- ligands are modulated by their differential sialylation that is also associated with glycoprotein positioning in membranes[8].
- is involved in lymphocyte trafficking and leukocyte recruitment[5][9].
Contents |
CFG Participating Investigators contributing to the understanding of this paradigm
CFG Participating Investigators (PIs) contributing to the understanding of galectin-1 include:
- Linda Baum
- C. Fred Brewer
- Richard Cummings
- Anne Dell
- Ten Feizi
- M.G. Finn
- Thomas Gerken
- Benhur Lee
- J. Michael Pierce
- Mauro Perretti
- Gabriel Rabinovich
- James Rini
- Sachiko Sato
- Gerald Schwarting
- Pamela Stanley
- Victor Thijssen
- Gerardo Vasta
- John Wang
Progress toward understanding this GBP paradigm
Carbohydrate ligands
Cellular expression
Structure
Biological roles of GBP-ligand interaction
CFG resources used in investigations
The best examples of CFG contributions to this paradigm are described below, with links to specific data sets. For a complete list of CFG data and resources relating to this paradigm, see the CFG database search results for Galectin-1.
Glycan profiling
Glycogene microarray
Knockout mouse lines
CFG-generated galectin-1 knockout mice have been used to study the biological functions of this paradigm GBP. The phenotype of galectin-1 knockout mice was analyzed by the CFG.
Glycan array
Investigators have used carbohydrate compounds and glycan microarrays to study ligand binding specificity of galectin-1.
Related GBPs
Galectins-2, -5, -7, -10, -11, -13, and -14
References
- ↑ Stowell SR, et al. Ligand reduces galectin-1 sensitivity to oxidative inactivation by enhancing dimer formation. J Biol Chem 284, 4989-4999 (2009).
- ↑ 2.0 2.1 Leppanen A, Stowell S, Blixt O, Cummings RD. Dimeric galectin-1 binds with high affinity to alpha2,3-sialylated and non-sialylated terminal N-acetyllactosamine units on surface-bound extended glycans. J Biol Chem 280, 5549-5562 (2005).
- ↑ Earl LA, Bi S, Baum LG. N- and O-glycans modulate galectin-1 binding, CD45 signaling, and T cell death. J Biol Chem 285, 2232-2244 (2010).
- ↑ Song X, et al. Novel fluorescent glycan microarray strategy reveals ligands for galectins. Chem Biol 16, 36-47 (2009).
- ↑ 5.0 5.1 Cooper D, Norling LV, Perretti M. Novel insights into the inhibitory effects of Galectin-1 on neutrophil recruitment under flow. J Leukoc Biol 83, 1459-1466 (2008).
- ↑ Stillman BN, et al. Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. J Immunol 176, 778-789 (2006).
- ↑ Dias-Baruffi M, et al. Differential expression of immunomodulatory galectin-1 in peripheral leukocytes and adult tissues and its cytosolic organization in striated muscle. Glycobiology In Press. (2010).
- ↑ Cha SK, et al. Removal of sialic acid involving Klotho causes cell-surface retention of TRPV5 channel via binding to galectin-1. Proc Natl Acad Sci U S A 105, 9805-9810 (2008).
- ↑ Norling LV, Sampaio AL, Cooper D, Perretti M. Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking. Faseb J 22, 682-690 (2008).
Acknowledgements
The CFG is grateful to the following PIs for their contributions to this wiki page: Linda Baum, Richard Cummings
